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Recipes
2.5% Cobalt Solution
-0.25 g CoCl2
-10 ml of distilled water
It may be stored for a long time
Ammonium Sulfide solution
-5 ml Ringer
-5 drops of ammonium sulfide,
concentrated ((NH4)2S)
Prepare when needed only.
Holder AAF
-80 ml 10%
ethanol -8 ml
glacial acetic acid
-12 ml 40%
formaldehyde It may be
stored for a long time
Revealing base solution
-100 ml distilled
water -3 g Arabic
glue
-10 g
glucose
-Shake well. Take to PH 2.5 - 2.6 with citric acid. It can
-0.17 g
hydroquinone be
stored for a few days in a fridge.
-1.4 g citric acid
Revealing solution (with
silver)
-10 ml revealing base solution
-1 ml AgNO3 1%
Prepare only when needed
Ringer solution
Base solution:
-1000 ml distilled
water -0.35 g KCl
-7.5 g
NaCl -0.21 g
CaCl2
-0.14 g
NaHCO3 -2.5 g glucose
take to PH 7.0
Buffer:
-A: solution 0.15 molar KH2PO4
-B: solution 0.15 molar Na2HPO4
For the buffer, mix 19 volumes of A
+ 31 volumes of B.
Ringer: 10 volumes of base solution
+ 1 volume of buffer
Bibliography
Insausti, T.C. (1994) Nervous
system of Triatoma infestans. Journal of Morphology 221: 343-359.
Insausti, T.C. & Lazzari, C.R.
(1996) Central projections of first-order ocellar interneurons in
Triatoma infestans (Heteroptera:Refuviidae). Journal of Morphology
229(2):161-169
Insausti, T.C. (1997) Estudio del
sistema ocelar de Triatoma infestans (Klug, 1834) (Heteroptera:
Reduviidae). Tesis de Doctorado en Ciencias Biologicas, F. CE.N. -
UBA, 259 pp.
Strausfeld N. J. Miller T. A. (eds)
Neuroanatomical techniques. Insect nervous system. Springer-Verlag,
1980.
Strausfeld N.J. (ed) functional
neuroanatomy. Springer-Verlag, 1983.
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