Bunonema sp.

CGC strain code:

PDL0026

Old codes:

WCUG9

Locality:

Forest leaf litter aside the driveway of the International Institute of Parasitology, St Albans, U.K. Collected on May 29th 1996 by David Hunt.

Classification:

Secernentea - Rhabditida - Rhabditina - Bunonematoidea - Bunonematidae

Culture conditions:

Xenic: Kept on "semi-wet plates": E. coli on 1% pure agar + 4 ml P-buffer pH 7.3 (removed again prior to nematode transfer) + 5 µg/ml cholesterol. Other bacteria hare not been removed.

Inoculation procedure:

E. coli are spread out over FRESH plates the day before transfer of nematodes as follows.

pipette 4 ml P-buffer (0.05M KH2PO4/K2HPO4 to pH 7.3) onto a 9 cm plate,
add 3 drops of E. coli with a narrow Pasteur pipette,
stir the plate gently, and then incubate the plate for a night at RT (this allows the E. coli to form a thin mat on the agar).
POUR OFF WATER the next day, and leave plates to evaporate on a flow bench for max. 30 mins (surface should still be fairly wet).
transfer Bunonema n.g. by washing off in P-buffer or by cutting agar with a cluster of adults from an old plate and applying it to the new plate.

Long-term storage:

Freezing was not attempted.

Availability:

Dead and Gone!!

Description:

None

Identification:

Paul De Ley.

Note:

No males. The classic Weird Worm of All Times, with asymmetrically warty cuticle.

Last revised: 27 March 1997