Reddish-brown loamy mud from an artificial marsh (private property, Heikens
10) in Beerzel, Belgium. No vegetation at sampling spot. Collected by Patsy
Scheldeman in October 1995.
Xenic: "Wet plates": 3-5 drops of E. coli in 4 ml P-buffer (0.05M
KH2PO4/K2HPO4 to pH 7.3) on 1% pure agar + 5 µg/ml cholesterol.
Inoculation procedure:
E. coli are suspended over FRESH plates the day before transfer
of nematodes as follows.
pipette 4 ml of P-buffer (pH 7.3) onto a 9 cm plate,
add 3-5 drops of E. coli with a narrow Pasteur pipette,
stir the plate gently, and then incubate the plate for a night at RT (this
allows the E. coli to form a thin mat on the agar).
Transfer a large number of specimens with a (glass) Pasteur pipette, or
transfer individual animals in 2 ml with a micropipette with non-sticky
tip (e.g. siliconized or glass tip drawn out as finely as possible and
welded onto a cut plastic tip with paraffin). AVOID USING NEEDLES.
Long-term storage:
Freezing has not been attempted.
Availability:
Freely available on "wet plates".
Description:
Andrássy, I. (1985) The genus Plectus Bastian, 1865 and its
nearest relatives (Nematoda: Plectidae). Acta Zoologica Hungarica,
31: 1-52.
Identification:
Paul De Ley.
Notes:
Males not yet seen. Relatively big adults; generation time estimated at
two weeks or more. S-buffer not recommended.
Last Updated: 27 March 1998
