Plectus sp. 2

CGC strain code:

PDL0013

Old codes:

WCUG13

Locality:

Very dark soil under leaf litter from a park forest (Mahy property) in Landegem, Belgium. Mixed deciduous trees (sample taken 2m from an oak). Collected by Paul De Ley in November 1994.

Classification:

Chromadoria - Chromadorida - Leptolaimina - Plectoidea - Plectidae

Culture conditions:

Xenic: "Wet plates": 3-5 drops of E. coli in 4 ml P-buffer (0.05M KH2PO4/K2HPO4 to pH 7.3) on 1% pure agar + 5 µg/ml cholesterol.

Inoculation procedure:

E. coli are suspended over FRESH plates the day before transfer of nematodes as follows.

pipette 4 ml of P-buffer (pH 7.3) onto a 9 cm plate,
add 3-5 drops of E. coli with a narrow Pasteur pipette,
stir the plate gently, and then incubate the plate for a night at RT (this allows the E. coli to form a thin mat on the agar).
Transfer a large number of specimens with a (glass) Pasteur pipette, or transfer individual animals in 2 ml with a micropipette with non-sticky tip (e.g. siliconized or glass tip drawn out as finely as possible and welded onto a cut plastic tip with paraffin). AVOID USING NEEDLES.

Long-term storage:

Freezing has not been attempted.

Availability:

It was never happy, and now it's gone.

Description:

None

Identification:

Paul De Ley.

Notes:

Males not yet seen. Big adults; reproduction fairly slow. Specimens are quite sensitive to pH and desiccation; perhaps also to salinity (S-buffer not recommended).

Last Updated: 27 March 1998