Plectus 1, Plectus 3, WCUG12, WCUG14, identical strain as PDL0014
Locality:
Very dark soil under leaf litter from a park forest (Mahy property) in
Landegem, Belgium. Mixed deciduous trees (sample taken 2m from an oak).
Collected by Paul De Ley in November 1994.
"Wet plates": 3-5 drops of E. coli in 4 ml P-buffer (0.05M KH2PO4/K2HPO4
to pH 7.3) on 1% pure agar + 5 µg/ml cholesterol.
Dry plates: 3 drops of E. coli on 1% pure agar + 5 µg/ml cholesterol.
Use fresh plates to make sure they still have a water film on the surface.
Inoculation procedure for wet plates:
E. coli are suspended over FRESH plates the day before transfer
of nematodes as follows.
pipette 4 ml of P-buffer (pH 7.3) onto a 9 cm plate,
add 3-5 drops of E. coli with a narrow Pasteur pipette,
stir the plate gently, and then incubate the plate for a night at RT (this
allows the E. coli to form a thin mat on the agar).
Transfer a large number of specimens with a (glass) Pasteur pipette, or
transfer individual animals in 2 ml with a micropipette with non-sticky
tip (e.g. siliconized or glass tip drawn out as finely as possible and
welded onto a cut plastic tip with paraffin). AVOID USING NEEDLES.
Long-term storage:
Freezing has not been attempted.
Availability:
Freely available on agar.
Description:
De Ley, P. & Coomans, A. (1994). Terrestrial nematodes from the Galápagos
Archipelago IV: The genus Plectus Bastian, 1865, with description
of three new species (Leptolaimina: Plectidae). Bulletin van het Koninklijk
Belgisch Instituut voor Natuurwetenschappen - Biologie, 64: 43-70.
Identification:
Paul De Ley.
Notes:
Males not yet seen. Small adults; reproduction relatively rapid, can reach
high densities. Specimens are quite sensitive to pH and desiccation; perhaps
also to salinity (S-buffer not recommended).
Last Updated: 27 March 1998
