Pseudodiplogasteroides n.sp.

CGC strain code:

PDL0008

Old codes:

Z7, WCUG8

Locality:

Sample from sand in coastal area. Nature reserve "Het Zwin", Belgium. Sample collected on 14 February 1994 and nematodes extracted by John Sudheer Midituri as a contaminant in Steinernema extraction with Galleria trap.

Classification:

Secernentea - Diplogasterida - Diplogasteroidea - Pseudodiplogasterioididae

Culture conditions:

Monoxenic: NA + 5mg/ml cholesterol + E. coli. Keep at 16C to room temperature.

Long-term storage:

Survives freezing in glycerin (low efficiency).

Availability:

Freely available on agar plates.

Descriptions:

Material was sent to Walter Sudhaus.

Identification:

Paul De Ley.

Note:

Males very rare, only found in crowded cultures, but actively mating. Generation time about 4 days. Chloroxing (NaOCl/KOH) kills animals but does not dissolve them efficiently.

Last Updated: 27 March 1998