Zeldia punctata (Thorne, 1925) Thorne, 1937

CGC strain code:

PDL0003

Other codes:

alphaD, WCUG3, JB-040

Locality:

Nebe, central Senegal: Sahel soil 5 km south of Diourbel along road Diourbel-Gossas. Collected in August 1991 by Pierre Baujard.

Classification:

Secernentea - Rhabditida - Cephalobina - Cephaloboidea - Cephalobidae

Culture conditions:

Xenic: E. coli on 1.5% pure agar (with or without 0.1% NA) + 5 µg/ml cholesterol. Currently kept on "wet plates" (1% agar + cholesterol + 5 ml 0.05M phosphate buffer at pH 7.3 + E. coli). No attempts to remove other bacteria have so far been made.

Long-term storage:

No survival after freezing in glycerin. Z. punctata can be stored 6-12 months on slowly desiccated plates. Dry slivers of such plates can be stored permanently by freezing them at -80°C. However, it is difficult in practice to achieve gradual desiccation of sufficient numbers of worms to guarantee proper recovery. Always check a sliver of the desiccated plate for recovery, both before and after freezing at -80°C.

Availability:

Freely available on agar.

Descriptions:

Allen, M.V. & Noffsinger, E.M. (1972). A review of the genus Zeldia Thorne, 1937 (Nematoda: Cephalobidae) with descriptions of seven new species. Proceedings of the Helminthological Society of Washington 39: 206-223. - Redescription of types & topotypes from California.

Rashid, F.; Geraert, E. & Sharma, R.D. (1985). Morphology, taxonomy and morphometry of some Cephalobidae (Nematoda: Rhabditida) from Brazil, with descriptions of two new genera and four new species. Nematologica 30 (1984): 251-298.

De Ley, P.; Geraert, E. & Coomans, A. (1990). Seven cephalobids from Senegal. Journal of African Zoology 104: 287-304.

Boström, S. (1992). Some Cephalobidae (Nematoda: Rhabditida) from Crete, Greece. Fundamental and Applied Nematology 15: 289-295 - with SEM.

Identification:

Paul De Ley.

Notes:

Do not keep below 20°C - thrives at 34°C according to Pierre Baujard! Parthenogenic. Multiplies moderately fast (for a cephalobid). Males not yet observed.

Last Updated: Tuesday 27 March 1998