Acrobeles complexus Thorne, 1925

CGC strain code:

PDL0002

Pictures:

Click here for an 87 Kb SEM picture of the lip region.

Other codes:

Z, WCUG2, JB-039

Locality:

Landegem, Belgium: sandy soil with broken herb cover (mostly grasses), from a field next to the "Schipdonk Canal" used as deposit site for sediment dredged out in 1980. Collected in 1990 by P. De Ley.

Classification:

Secernentea - Rhabditida - Cephalobina - Cephaloboidea - Cephalobidae

Culture conditions:

Xenic: E. coli on 1-1.5% pure agar (with or without 0.1% NA) + 5 µg/ml cholesterol. Currently kept on "wet plates" (1% agar + cholesterol + 5 ml 0.05M phosphate buffer at pH 7.3 + E. coli). No attempts to remove other bacteria have so far been made. Kept at room temperature (never below 20C).

Long-term storage:

No survival after freezing in glycerin. Can be stored 6-12 months on slowly desiccated plates; such plates can be stored permanently in liquid nitrogen.

Availability:

Freely available on agar.

Descriptions:

Thorne, G. (1961). Principles of Nematology. McGraw-Hill, New York. 553 pp. - S. complexa described on p. 472.

Thomas, P.W. (1965). Morphology of Acrobeles complexus Thorne cultivated on agar. Nematologica 11: 383-394.

Rashid, F.; Heyns, J. & Coomans, A. (1990). Species of Seleborca Andrássy, 1985 from South West Africa/Namibia (Nematoda: Cephalobidae). Phytophylactica 22: 51-62. - with SEM.

Boström, S. (1992). Some Cephalobidae (Nematoda: Rhabditida) from Crete, Greece. Fundamental and Applied Nematology 15: 289-295 - with SEM.

Identification:

Paul De Ley.

Notes:

Autotokous. Multiplies slowly (hence culture on pure agar preferred over NA or NGM) but can last over a year in well-sealed plates.

Last Updated: Monday 01 Nov. 2004