Research in Bill Walton's Laboratory
Research and Publications: I am an aquatic entomologist and ecologist who works mostly in natural and man-made wetlands. Currently, the research in my laboratory has two foci: wetlands ecology with an emphasis on mosquitoes and bacteria used as an environmentally friendly method for controlling mosquitoes. Links to recent publications can be found either in the links in the paragraphs below or in the list of publications link at the bottom of this section of the web page.
The major emphasis of the wetlands research in my laboratory group is to integrate studies of mosquito biology and ecology with the design of control methodologies for pestiferous and pathogen-transmitting mosquitoes in wetlands. We have been studying the effects of design features and management strategies for multipurpose constructed treatment wetlands on mosquito production and water quality performance. In addition to studying the relationship of nutrients and other water quality variables to mosquito populations, our studies have focused across the food web from the temporal and spatial dynamics of bacterial populations to studies on alternative larvivorous fishes to the mosquitofish (Gambusia affinis) and collaborative studies with USGS scientists on the relationship of aquatic insect production to bird usage of wetland habitats. Our applied research on mosquito control in man-made wetlands has been complemented with basic research on the population dynamics of larval mosquito populations, the ecological stoichiometry of mosquitoes, the interaction of mosquitoes with natural enemies, and the influence of vegetation on the temporal and spatial distribution of mosquito larvae in wetlands. More recently, we have been studying the efficacy of alternative emergent macrophytes for constructed treatment wetlands, the microbiomes of mosquitoes and their environments, and the genetic/genomic basis of host selection of mosquitoes.
A second research emphasis in my laboratory is the
evolution of resistance to bacterial larvicides and the ecological consequences
of evolved resistance. Bacterial larvicides are perhaps the most promising method of environmentally
friendly mosquito control currently available, particularly in treatment
wetlands. Two bacteria species are
currently used for mosquito control in California; Lysinibacillus sphaericus is
comparatively more effective than is Bacillus
thuringiensis subsp. israelensis
(Bti) against mosquitoes inhabiting the
organically enriched waters of most treatment wetlands. Unlike Bti which contains multiple
toxins that limit the potential for the rapid evolution of resistance in
mosquitoes, the two toxin precursors in L.
sphaericus act as a single toxin following ingestion and partial digestion
by mosquito larvae. Mosquitoes can
evolve resistance to the L. sphaericus toxin very rapidly (>10,000-fold in 7-8
generations). This finding
We have been collaborating with Dr. Brian Federici and his laboratory on an NIH-funded project studying the development, modes of action and spectra of resistance/cross-resistance of transgenic strains of Bacillus that have been engineered for both increased efficacy against mosquitoes and a reduced potential for the evolution of resistance in the target mosquito populations. Dr. Margaret Wirth, who recently retired from UCR, coordinated this work in my laboratory and, in addition to her considerable expertise in studies of insect resistance, oversaw the maintenance of 22 colonies of mosquitoes that were selected for resistance to various Bacillus toxins and insecticides. We also have collaborated on related projects with colleagues at the Pasteur Institute in Paris, Ohio State University, Cardiff University and Ben-Gurion University of the Negev. The findings of these studies have important implications for genetic engineering of bacterial larvicides and resistance management in programs using bacterial larvicides as an environmentally-friendly approach to mosquito control and for reducing the incidence of mosquito-borne disease in developing nations.
Chan and Julia Huynh quantify spore germination rates prior to application
of fungi in underground storm drains. Thu
McLaughlin and Mostafa Azadbakht
assess chlorophyll biomass in samples from a mesocosm
Greer filters water samples from experimental wetlands.
Vivian Chan and Julia Huynh quantify spore germination rates prior to application of fungi in underground storm drains.
Thu McLaughlin and Mostafa Azadbakht assess chlorophyll biomass in samples from a mesocosm experiment.
Jordan Greer filters water samples from experimental wetlands.
Undergraduate students have participated in many of the research projects in the laboratory. Some of the recent undergraduate collaborators are shown above.
Other recent foci of work in my laboratory have been (1) investigating the use of biorational agents as an environmentally friendly method of controlling mosquitoes inhabiting underground storm drain systems (USDS), (2) the development of alternative methods for the surveillance of mosquito vectors and the pathogens that they transmit and (3) identification of the semiochemicals released by potential predators of mosquitoes that are detected by egg-laying mosquitoes to alter oviposition behavior.
We have been working in USDS in the Coachella Valley investigating the persistence and efficacy of commercially available formulations of fungi that are used by organic farmers against a wide variety of insect pests. These same strains of insect-specific fungi are effective against Cx. quinquefasciatus, a mosquito commonly found in USDS and an important vector of the West Nile virus in urban and suburban areas of southern California. Our preliminary findings indicate that meaningful levels of infection can persist in USDS for a month or more, and infections were detected in bioassays for up to 11 weeks after application in the field. This work has been supported by the Coachella Valley Mosquito and Vector Control District, the Mosquito Research Foundation and the USDA-NIFA through the Agricultural Experiment Station at U.C. Riverside.
Our work on methods for the enhanced surveillance of vectors and mosquito-borne pathogens has lead to the development of two gravid traps that, on average, collect 7-fold more mosquitoes than does the standard CDC gravid trap used routinely by vector control districts for vector surveillance. Even more important is that the new gravid traps collect vector mosquitoes that are rarely collected by the standard gravid trap. For example, during spring, one of the new gravid trap designs collected up to nearly 70-fold more Cx. tarsalis than did the standard gravid trap and, on some dates, collected Cx. tarsalis and Cx. stigmatosoma when the CDC gravid trap failed to do so. We investigated the relative importance of various components of the trap for collecting Cx. quinquefasciatus, Cx. stigmatosoma and Cx. tarsalis. While the standard CDC gravid trap is still a more portable and probably a more practical method of collecting mosquitoes in the Cx. pipiens/Cx. quinquefasciatus species group than are the new designs, our alternative gravid trap designs may enhance the detection of arbovirus presence in vectors early in the annual activity period of mosquitoes. Intervention at this time is critical to preventing amplification of arbovirus infections that can decimate particular species of wildlife and can spill over into humans and companion animals. A manuscript describing the two most effective designs will appear soon.
We found in both the laboratory and field that water containing chemicals released by fish used as biological control agents against mosquitoes can be detected by egg-laying females of some mosquitoes. The numbers of egg rafts laid by Cx. tarsalis on water that previously contained mosquitofish (Gambusia affinis) were 80-90% lower than on water that was not exposed to potential larvivores. Not surprisingly, mosquito species, such as Aedes aegypti, that lay eggs in small containers did not respond differentially to fish-conditioned water. Adena Why is following up these studies by investigating the responses of egg-laying mosquitoes to other potential predators of mosquitoes and by identifying the semiochemicals that elicit responses in gravid mosquitoes.
005C, Introductory Evolution and Ecology
BIOL 265, Advances in Population and Evolutionary Biology
ENTM 114, Aquatic Insects
ENTM 127, Insect Ecology
ENTM 203, Entomology Graduate Core Curriculum: Supraorganismal Disciplines
ENTM 255, Seminar in Medical and Veterinary Entomology
ENTM 276, Research Seminar in Medical, Urban and Veterinary Entomology
David Popko, M.S.
Current Graduate Students:
Nathan McConnell (Entomology), Ph.D. candidate
Steven Merkley (EEOB), Ph.D. candidate
Adena Why (Entomology), Ph.D. candidate
Current Undergraduate Students:
The Lab Group, summer 2010. Front row (left to
right): Dagne Duguma, Adena Why, Andrew Nguyen, Sammy Wong, Peggy Wirth,
Justin Richardson Back row: David Popko, Kevin Mai, Tristan Hallum,
Bill Walton, Jennifer Henke,
Missing: Donald Beasley, Henrique Chan, Bich
Nguyen, Jennifer Thieme, Rex Tse
The Lab Group, summer 2010. Front row (left to right): Dagne Duguma, Adena Why, Andrew Nguyen, Sammy Wong, Peggy Wirth, Justin Richardson Back row: David Popko, Kevin Mai, Tristan Hallum, Bill Walton, Jennifer Henke, Missing: Donald Beasley, Henrique Chan, Bich Nguyen, Jennifer Thieme, Rex Tse
Prospective Graduate Students:
I am most interested in working with students who have a strong interest in the study of aquatic insect ecology or vector ecology. Besides serving on committees of graduate students enrolled in the Entomology Graduate Program, I have recently served on the qualifying exam, thesis or dissertation committees of students in the departments of Biology and Environmental Sciences. I am a Cooperating Faculty Member in the Evolution, Ecology and Organismal Biology Graduate Program. I am associated with the Center for Conservation Biology, the Center for Invasive Species Research and the Center for Disease Vector Research. Prospective students are welcome to contact me before applying to best determine which program is most appropriate for their interests and prior training.