CGC strain code:
Forest leaf litter aside the driveway of the International Institute of
Parasitology, St Albans, U.K. Collected on May 29th 1996 by David Hunt.
Secernentea - Rhabditida - Rhabditina - Bunonematoidea - Bunonematidae
Xenic: Kept on "semi-wet plates": E. coli on 1% pure agar + 4 ml
P-buffer pH 7.3 (removed again prior to nematode transfer) + 5 µg/ml
cholesterol. Other bacteria hare not been removed.
E. coli are spread out over FRESH plates the day before transfer
of nematodes as follows.
pipette 4 ml P-buffer (0.05M KH2PO4/K2HPO4 to pH 7.3) onto a 9 cm plate,
add 3 drops of E. coli with a narrow Pasteur pipette,
stir the plate gently, and then incubate the plate for a night at RT (this
allows the E. coli to form a thin mat on the agar).
POUR OFF WATER the next day, and leave plates to evaporate on a flow bench
for max. 30 mins (surface should still be fairly wet).
transfer Bunonema n.g. by washing off in P-buffer or by cutting
agar with a cluster of adults from an old plate and applying it to the
Freezing was not attempted.
Dead and Gone!!
Paul De Ley.
No males. The classic Weird Worm of All Times, with asymmetrically warty
Last revised: 27 March 1997