Xenic: "Wet plates": 3-5 drops of E. coli in 4 ml P-buffer (0.05M
KH2PO4/K2HPO4 to pH 7.3) on 1% pure agar + 5 µg/ml cholesterol.
E. coli are suspended over FRESH plates the day before transfer
of nematodes as follows.
pipette 4 ml of P-buffer (pH 7.3) onto a 9 cm plate,
add 3-5 drops of E. coli with a narrow Pasteur pipette,
stir the plate gently, and then incubate the plate for a night at RT (this
allows the E. coli to form a thin mat on the agar).
Transfer a large number of specimens with a (glass) Pasteur pipette, or
transfer individual animals in 2 ml with a micropipette with non-sticky
tip (e.g. siliconized or glass tip drawn out as finely as possible and
welded onto a cut plastic tip with paraffin). AVOID USING NEEDLES.
Freezing has not been attempted.
Freely available on "wet plates".
Andrássy, I. (1985) The genus Plectus Bastian, 1865 and its
nearest relatives (Nematoda: Plectidae). Acta Zoologica Hungarica,
Paul De Ley.
Males not yet seen. Relatively big adults; generation time estimated at
two weeks or more. S-buffer not recommended.
Last Updated: 27 March 1998