Xenic: "Wet plates": 3-5 drops of E. coli in 4 ml P-buffer (0.05M
KH2PO4/K2HPO4 to pH 7.3) on 1% pure agar + 5 µg/ml cholesterol. Keep
E. coli are suspended over FRESH plates the day before transfer
of nematodes as follows.
pipette 4 ml of P-buffer (pH 7.3) onto a 9 cm plate,
add 3-5 drops of E. coli with a narrow Pasteur pipette,
stir the plate gently, and then incubate the plate for a night at RT (this
allows the E. coli to form a thin mat on the agar).
Transfer a large number of specimens with a (glass) Pasteur pipette, or
transfer individual animals in 2 ml with a micropipette with non-sticky
tip (e.g. siliconized or glass tip drawn out as finely as possible and
welded onto a cut plastic tip with paraffin).
Freezing has not been attempted.
Freely available on "wet plates" which contain a contaminant fungus.
Paul De Ley.
Males not yet seen. Small hermaphrodites (?) with denticles on glottoid
apparatus, small amphid posterior to lips, long tail and two gonads; reproduction
moderately rapid. S-buffer not recommended.
Last Updated: 27 March 1998