Plectus sp. 2
CGC strain code:
Very dark soil under leaf litter from a park forest (Mahy property) in
Landegem, Belgium. Mixed deciduous trees (sample taken 2m from an oak).
Collected by Paul De Ley in November 1994.
Chromadoria - Chromadorida - Leptolaimina - Plectoidea - Plectidae
Xenic: "Wet plates": 3-5 drops of E. coli in 4 ml P-buffer (0.05M
KH2PO4/K2HPO4 to pH 7.3) on 1% pure agar + 5 µg/ml cholesterol.
E. coli are suspended over FRESH plates the day before transfer
of nematodes as follows.
pipette 4 ml of P-buffer (pH 7.3) onto a 9 cm plate,
add 3-5 drops of E. coli with a narrow Pasteur pipette,
stir the plate gently, and then incubate the plate for a night at RT (this
allows the E. coli to form a thin mat on the agar).
Transfer a large number of specimens with a (glass) Pasteur pipette, or
transfer individual animals in 2 ml with a micropipette with non-sticky
tip (e.g. siliconized or glass tip drawn out as finely as possible and
welded onto a cut plastic tip with paraffin). AVOID USING NEEDLES.
Freezing has not been attempted.
It was never happy, and now it's gone.
Paul De Ley.
Males not yet seen. Big adults; reproduction fairly slow. Specimens are
quite sensitive to pH and desiccation; perhaps also to salinity (S-buffer
Last Updated: 27 March 1998