Teratocephalus lirellus Anderson, 1969
CGC strain code:
Click here for a 89 Kb SEM picture of the lip
Click here for a 175 Kb TEM picture of the
Very dark soil under leaf litter from a park forest (Mahy property) in
Landegem, Belgium. Mixed deciduous trees (sample taken 2m from an oak).
Collected by Paul De Ley in November 1994.
Secernentea - Rhabditida - Teratocephalina - Teratocephalidae
Xenic: "Wet plates": 3-5 drops of E. coli in 4 ml P-buffer (0.05M
KH2PO4/K2HPO4 to pH 7.3) on 1% pure agar + 5 µg/ml cholesterol. Keep
E. coli are suspended over FRESH plates the day before transfer
of nematodes as follows.
pipette 4 ml of P-buffer (pH 7.3) onto a 9 cm plate,
add 3-5 drops of E. coli with a narrow Pasteur pipette,
stir the plate gently, and then incubate the plate for a night at RT (this
allows the E. coli to form a thin mat on the agar).
Transfer a large number of specimens with a (glass) Pasteur pipette, or
transfer individual animals in 2 ml with a micropipette with non-sticky
tip (e.g. siliconized or glass tip drawn out as finely as possible and
welded onto a cut plastic tip with paraffin). AVOID USING NEEDLES.
Freezing has not been attempted.
Freely available on agar.
Anderson, R.V. (1969). Comparative morphology and descriptions of three
new species of Teratocephalus from Canada. Canadian Journal of Zoology,
47: 829-840. Karegar, A.; De Ley, P. & Geraert, E. (in press). Three
teratocephalid nematodes from Iran. Fundamental and Applied Nematology,
Paul De Ley.
Males not yet seen. Reproduction is slow (1 egg per female per week? 2
weeks generation time?), but massive numbers can build up in well-sealed
plates left alone for three to four months after inoculating abundantly.
Specimens are quite sensitive to pH and desiccation; perhaps also to salinity
(S-buffer not recommended). Feeding extra E. coli is not necessary
for build-up and even results in nematode mortality (pH-shock?).
Last Updated: 27 March 1998