Xenic: Kept on "semi-wet plates": E. coli on 1% pure agar + 4 ml
P-buffer pH 7.3 (removed again prior to nematode transfer) + 5 µg/ml
cholesterol. Other bacteria were not been removed (may even have been essential).
Keep at 16-18C.
E. coli are spread out over FRESH plates the day before transfer
of nematodes as follows.
pipette 4 ml P-buffer (0.05M KH2PO4/K2HPO4 to pH 7.3) onto a 9 cm plate,
add 3 drops of E. coli with a narrow Pasteur pipette,
stir the plate gently, and then incubate the plate for a night at RT (this
allows the E. coli to form a thin mat on the agar).
POUR OFF WATER the next day, and leave plates to evaporate on a flow bench
for max. 30 mins (surface should still be fairly wet).
transfer Myolaimus by washing off in P-buffer or by cutting agar
with a cluster of adults from an old plate and applying it to the new plate.
This probably transfers some necessary unknown bacteria, because inoculations
with needle-picked individuals usually fail.
Freezing was not attempted.
Dead and Gone!!
Cobb, N.A. (1920). One hundred new nemas (type species of 100 new genera).
Contributions to a Science of Nematology, 9: 217-343.
Paul De Ley.
Males common, probably amphimictic. Animals seem to be unhappy on the surface;
soft gels recommended to allow them to dig in.
Last Updated: 27 March 1998